Journal: Gene therapy

Article Title: Electroporation-mediated Delivery of FER Gene Enhances Innate Immune Response and Improves Survival in a Murine Model of Pneumonia

doi: 10.1038/s41434-018-0022-y

Figure Lengend Snippet: 500 CFU of Klebsiella pneumoniae was given to C57/Bl6 female mice. 150 μg of plasmid DNA containing gene encoding human tyrosine kinase FER was given either one hour prior (solid triangle) or after (solid square) insult and animals were electroporated using a BTX ECM 830 generator receiving eight 200 V cm −1 square wave pulses,10 ms duration and 1 second apart. Sham electroporated with saline solution (EP-saline) and EP of empty vector ( p cDNA 3.1) served as controls. Both protective and therapeutic strategies of EP-mediated FER gene transfer showed improved survival over controls. N=10 mice per group. Significance placed in graph. Log-rank (Mantel-Cox) test.

Article Snippet: The original pSG5-FER plasmid was a gift from Nora Heisterkamp deposited in Addgene DNA repository (Addgene plasmid # 30191).

Techniques: Plasmid Preparation

Journal: Scientific Reports

Article Title: Chromobacterium spp . mediate their anti- Plasmodium activity through secretion of the histone deacetylase inhibitor romidepsin

doi: 10.1038/s41598-018-24296-0

Figure Lengend Snippet: Bacterial strains in this study.

Article Snippet: C. pseudoviolaceum , LMG 3953 , DSMZ (DSM 23279) , CPSE , .

Techniques:

Journal: Scientific Reports

Article Title: Chromobacterium spp . mediate their anti- Plasmodium activity through secretion of the histone deacetylase inhibitor romidepsin

doi: 10.1038/s41598-018-24296-0

Figure Lengend Snippet: Anti- Plasmodium activity of chromobacteria is restricted to the romidepsin-producing subcluster comprised of C. sp. Panama and C. haemolyticum . ( A ) Variation in growth of asexual stage P. falciparum NF54 upon incubation with n -butanol extracts of approximately 5 mL of culture supernatants of different Chromobacterium species (LB, 72 h, 30 °C). 0% inhibition is adjusted for parasite growth in vehicle control (1% DMSO) and 100% inhibition is matched to that of 250 nM chloroquine. Results are shown as mean ± standard deviation; significance was determined using a one-tailed one sample t-test to determine whether each treatment significantly lowered parasite growth compared to control (ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001). CQ, chloroquine. CAQU, C. aquaticum ; CHAE, C. haemolyticum ; CPIS, C. piscinae ; CPSE, C. pseudoviolaceum ; CSPP, C. sp . Panama; CSUB, C. subtsugae ; CVAC, C. vaccinii ; CVIO, C. violaceum ; CW10, C. haemolyticum W10. ( B ) UPGMA tree generated from a distance matrix inferred from pairwise genome average nucleotide identity (gANI). A distance of 0.02 indicates that the gANI of two species differs by 2 percent points. Cophenetic Correlation Coefficient (CP) = 0.997961877789881. ( C ) Alignment between the reference romidepsin biosynthetic gene cluster in C. sp . 968 (C968; GenBank: EF210776.1) to the ones present in the genomes of C. sp . Panama (CSPP) and C. haemolyticum , both MDA0585 (CHAE) and W10 (CW10) strains. Geneious alignment in Geneious v5.4 (global alignment with free end gaps; cost matrix: 65% similarity).

Article Snippet: C. pseudoviolaceum , LMG 3953 , DSMZ (DSM 23279) , CPSE , .

Techniques: Activity Assay, Incubation, Inhibition, Standard Deviation, One-tailed Test, Generated

Journal: Scientific Reports

Article Title: Chromobacterium spp . mediate their anti- Plasmodium activity through secretion of the histone deacetylase inhibitor romidepsin

doi: 10.1038/s41598-018-24296-0

Figure Lengend Snippet: Sources of genome sequences of the Chromobacterium species/strains analyzed in this study.

Article Snippet: C. pseudoviolaceum , LMG 3953 , DSMZ (DSM 23279) , CPSE , .

Techniques: